Investigating antimicrobial properties of plants

Note 2 d Explain or demonstrate how to use Whatman antibiotic assay paper discs to assess microbial action. Divide the base into four sections by drawing a cross with the marker pen.

Cassia fistula, in vitro antibacterial activity, antifungal activity, secondary metabolites INTRODUCTION Antibiotics are one of our most important weapons in fighting bacterial infections and have greatly benefited the health-related quality of human life since their introduction.

There is evidence that Neanderthals living 60, years ago in present-day Iraq used plants such as hollyhock; these plants are still widely used in ethnomedicine around the world. In addition, many people are interested in having more autonomy over their medical care. If the extract contains ethanol, make sure it has evaporated completely before using.

Crush 3 g of plant material with 10 cm3 of ethanol IDA. If your students investigate the antimicrobial properties of essential oils, ensure that none are ingested — many are toxic in concentrated form.

Plant Products as Antimicrobial Agents

Its activity may be affected by factors such as dilution, temperature, pH, and the presence of detergent or organic matter. Reported to have antiseptic properties, they were even employed as mouthwashes.

They need to be aware of what to do with spills and how to dispose of cultures and contaminated equipment. Speculations as to how and why a selected number of plant species came into use for either food or drugs is fascinating but outside the scope of this review.

Any given antiseptic is usually more effective against some microbes than others. In the West, the Renaissance years saw a revival of ancient medicine, which was built largely on plant medicinals.

A technique for serial dilution is given in Standard techniques which could be used to make dilutions of a range of branded anti-microbials for testing. In general, bacteria have the genetic ability to transmit and acquire resistance to synthetic drugs which are utilized as therapeutic agents.

Soak the paper discs in the extract or solution to be tested. A piece of squared paper under the agar plate might be helpful here.

Some strains of the bacteria listed above have been associated with health hazards. During the Dark Ages, the Arab world continued to excavate their own older works and to build upon them.

Recognised educational suppliers should provide safe strains.

Investigating anti-microbial action

Among Europeans living in the New World, the use of botanicals was a reaction against invasive or toxic mainstream medicinal practices of the day. Consider the value of using a control disc soaked in ethanol and dried as above.

In many developing countries, traditional medicine is one of the primary healthcare systems. Clinical microbiologists have two reasons to be interested in the topic of antimicrobial plant extracts.Investigating antimicrobial properties of plants.

It's a common ingredient in chai tea, and it is believed to improve the digestion of fruit, milk and other dairy products. Mint Figure 3: Mint leaves (Fresh Mint) Mint has been used in healing remedies for many centuries.

1. discuss the importance of plant extracts as antimicrobials. 2. identify natural compounds found with antimicrobial properties in plants. 3. perform a scientific investigation on plants with possible antimicrobial activity.

4. use microbiological skills such as aseptic techniques and proper pouring of plates. Investigating the antibacterial properties of plants: Introducing STEM Careers This resource is part of the SAPS Careers in Science series, a set of resources to support science teachers in integrating STEM careers information with practical activities.

Antimicrobial Properties of Plants A plant with antimicrobial properties means that it destroys or inhibits the growth of bacteria and fungi. Antimicrobial Properties Plants are susceptible to infection by bacteria and fungi.

An antimicrobial is a substance that inhibits or destroys the growth of certain bacteria. Several plants are known to, or thought to have this antimicrobial property to repel attacks by microorganisms.5/5(1).

Investigation into the antibacterial properties of mint and garlic.

Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: An ethnomedicinal plant The antibacterial and antifungal activities of extracts (5, 25, 50,μg/ml) Antimicrobial properties of medicinal plants are being increasingly reported from different parts of the world.

Investigating antimicrobial properties of plants
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