Screening and identification of rhizobacteria isolated

Statistical analysis Experiments were repeated twice, unless otherwise stated. The diazotrophic nature of all the recovered isolates was determined by ARA. Given the ability of diazotrophs to fix N, some strains may relieve N-deficiencies where there is inadequate application of N fertilizers.

Seed coating took place in a plastic bag. No use, distribution or reproduction is permitted which does not comply with these terms. Introduction of plant growth promoting rhizobacteria PGPR including phosphate solubilizing bacteria PSB as biofertilizers is suggested as a sustainable option for the improvement of nutrient availability, plant growth, and yields Vessey, Flasks were inoculated with bacteria previously grown in TSA for 48 h.

For example, Abiala et al. Some diazotrophic bacteria including those in the genera: Ninety-two isolates were selected for further screening because they were able to grow on N-free media containing different carbon sources. Acetylene reduction assay ARA The basis for the assay is the fact that nitrogenase, the enzyme complex in diazotrophic microorganisms that reduces nitrogen to ammonia, also reduces acetylene to ethylene.

Plates with NF medium with mannitol as a carbon source for diazotrophic bacteria were inoculated with 0. Easy and rapid molecular techniques can be developed to perform microbial characterization.

Combination of in vitro and in vivo screening may lead to identification of effective strains for sustainable agriculture. Studies confirmed that the positive effect of some diazotrophic bacterial species on non-leguminous plant yields may not only be due to nitrogen fixation but other mechanisms may also contribute to the growth responses observed in non-leguminous plants Caballero-Mellado et al.

Measurements were made on eight leaves of each pot of nine plants per treatment. Data were analyzed using GenStat executable release 14th edition statistical analysis software.

The isolates were screened in vitro for diazotrophic potential tests for ammonia production and acetylene reduction. After 5 days of incubation, colonies were transferred onto fresh N-free media, and after 2 days were streaked out onto tryptone soy agar TSA; Merck plates.

No use, distribution or reproduction is permitted which does not comply with these terms. The bag was shaken for 2 min, until all the seeds were uniformly wetted with the sticker suspension. The use of indigenous PGPR can be an added advantage since it can easily acclimatize to the natural conditions and enhance the plant—microbe interactions Verma et al.

Development of a brown to yellow color was a positive test for ammonia production.

The 16S rRNA gene sequencing technique was used for molecular identity and authentication. Isolates that showed significant effect on at least two growth parameters were identified at species or genera level.

Nitrogen is the most limiting nutrient for increasing crop productivity. The liquid was removed, and the pellet was briefly re-spun followed by the removal of residual ethanol.

Table 1 Physico-chemical properties of soil samples collected for isolation studies from different sites of maize growing areas in Hajira, Rawalakot, Azad Jammu and Kashmir, Pakistan.

Each treatment consisted of three pots with a top diameter of mm that held 2 kg of composted pine bark. Abstract Poor soil fertility is one of the major constraints for crop production.Screening of rhizobacteria isolated from maize (Zea mays L.) The identification of the genera through analyses of the sequences of 16S rDNA classified the isolates to the following six Screening of rhizobacteria that show multiple PGP traits suggests that they have better potential for greenhouse and field testing and applications in.

Screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability. bacteria were isolated on nutrient agar from root washing solutions (61 isolates), surface sterilized roots Since screening these microbial biodiversity and solubilizing Rhizobacteria The identification of the strains was performed according to their morphological, cultural, and biochemical.

Rhizobacteria traits in bacteria isolated and characterized from Perseabombycinarhizospheric soils. The present Screening and identification of traits in plant growth promoting rhizobacteria from rhizospheric. Eight PGPR strains isolated from the screened isolates (HJR 1, HJR 2, Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities.

Identification of rhizobacteria from maize and determination of their plant-growth promoting potential. Screening of soil rhizobacteria isolated from wheat plants grown in the Marrakech region (Morocco, North Africa) for plant growth promoting activities The rhizobacteria were isolated using a dilution plate technique [17] and the pure culture isolates were preserved Molecular identification.

Screening and identification of rhizobacteria isolated
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